Application of the [3.super.H]Leucine incorporation technique for quantification of bacterial secondary production associated with decaying wetland plant litter

Article Abstract:

The application of the [super.3.H]Leucine incorporation procedure for measuring bacterial production on decaying wetland plant litter is assessed by conducting substrate saturation experiments (nine concentrations, 10 nM to 50muM final leucine concentration) on three dates for microbial communities colonizing the submerged litter of three emergent plant species. The results reveal differential uptake by litter associated microbial assemblages, with the lower apparent [K.sub.m] values possibly representing bacterial uptake and higher apparent [K.sub.m] values representing a combination of both bacterial and nonbacterial uptake.

author: Francoeur, Steven N., Neely, Robert K., Gillies, Jane E., Kuehn, Kevin A.
Science & research, Environmental aspects, Leucine, Wetlands, Microbial ecology

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Osmoregulatory responses of fungi inhabiting standing litter of the freshwater emergent macrophyte Juncus effusus

Article Abstract:

The impact of increased dessication on the osmoregulatory response of fungal decomposers inhabiting standing litter of the macrophyte Juncus effusus was investigated under field and laboratory conditions. The concentrations of osmolytes in fungal decomposers were negatively correlated with plant litter water potentials and rates of microbial respiration under field conditions. Results suggested that fungal inhabitants associated with standing litter of macrophytes can adjust their intracellular solute concentrations in response to daily fluctuations in water availability.

author: Suberkropp, Keller, Kuehn, Kevin A., Churchill, Perry F.
Fungi, Fluid-electrolyte balance, Osmoregulation

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New spatially explicit method for detecting extracellular protease activity in biofilms

Article Abstract:

Research has been conducted on the protease activity at biofilm-substratum interfaces. The method of detecting the extracellular protease activity at these interfaces has been developed and described.

author: Francoeur, Steven N., Wetzel, Robert G., Neely, Robert K.
Statistical Data Included, Analysis, Proteases, Microbial mats, Microbiological research

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subjects list: Research, United States, Physiological aspects
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